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Poster: Growth Regulators and Hormones

820:Do plant cells have the receptor for acetylcholine? Immunodetection of acetylcholinesterase and acetylcholine receptor in leaf cells

Authors:Madhavan, S.(A)Pinkerton, Scott, T.(A)
Affiliations:(A): University of Nebraska
Presenter:Madhavan, S. , msoundar@unlinfo.unl.edu

Though it still lacks a clearly defined role in plants, acetylcholine (ACh), which is present in chloroplasts and other parts of the plant body, has been suggested to be a second messenger in various cellular processes. Acetylcholinesterase (AChE), the ACh hydrolyzing enzyme, has been well studied in animal systems. Different tissues of plants and their organelles are known to have AChE activity. In an earlier study (Madhavan et al., Plant Science, 107: 119-127, 1995) we reported the presence of a true AChE activity in stomatal guard cells. In the present study, we report the immunolocalization of AChE in guard cells and chloroplasts of Vicia faba leaf. Using monoclonal anti-AChE antibody we were able to localize AChE, in situ , studies, that AChE The first direct observation indicating the existence of ACh receptors, especially nAChR, in isolated protoplasts from etiolated wheat leaves, was made by Tretyn and Kendrick (1991, unpublished). Our preliminary studies, using monoclonal antibodies raised against the delta subunit of the n-AChR indicate that ~80 kD polypeptide band cross reacts with this antibody in the leaf extracts of Pisum sativum, Nicotiana tabacum, Vicia faba, and Triticum aestivum , but not in Zea mays . Extracts of isolated pea chloroplasts also exhibited cross reactivity to the 80 kD band. This observation supports the earlier finding of Tretyn and Kendrick on the presence of n-AChR in plant cells. Additionally, results from our studies on the immunolocalization of n-AChR in leaf cells will also be presented.

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