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Poster: Lipids and Related Molecules

263:Specific inhibition of isoprenoid (carotenoid, chlorophyll and isoprene) biosynthesis in plants by fosmidomycin.

Authors:Lichtenthaler, Hartmut, K.(A)Mueller, Christian(A)Schwender, Joerg(A)Jomaa, Hassan(B)Zeidler, Johannes(A)
Affiliations:(A): University of Karlsruhe, Botanical Institute II
(B): Justus-Liebig University, Academic Hospital Centre
Presenter:Lichtenthaler, Hartmut K., hartmut.lichtenthaler@bio-geo.uni-karlsruhe.de

Plastidic isoprenoids such as carotenoids, phytol, the prenyl-side chain of plastoquinone-9 or isoprene are made via the 1-deoxy-d-xylulose 5-phosphate (DOXP) pathway of IPP biosynthesis (Ann. Rev. Plant Physiol. Plant Molec. Biol. 50, 47-65, 1999). We have checked various chemical compounds as potential inhibitors of this non-mevalonate IPP and isoprenoid pathway. We found that the antibiotic fosmidomycin inhibits carotenoid, chlorophyll and isoprene biosynthesis in different plants as is reported here. The formation of isoprenoid pigments in ripening tomatoes (lycopene), etiolated barley shoots and duckweed plantlets was inhibited by fosmidomycin at concentrations of ca. 10 ÁM. The light-stimulated isoprene emission from leaf pieces of Populus nigra, Platanus x acerifolia and Chelidonium majus was inhibited by concentrations of fosmidomycin down to 1ÁM. Fosmidomycin did not affect the first enzyme of the DOXP pathway, the DOXP synthase. However, 1-deoxy-D-xylulose-5-phosphate reductoisomerase (DRI), the second enzyme of the non-mevalonate IPP pathway, was specifically inhibited by fosmidomycin (1ÁM) as measured in barley leaf protein extracts. The results demonstrate that fosmidomycin inhibits plastidic isoprenoid biosynthesis by blocking the enzyme DRI.

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