Poster: Abiotic Stress

Abs # 373: NaCl induced changes in ionically bound peroxidase activity in roots of rice seedlings.

Presenter:	Kao, Ching-Huei , kaoch@ccms.ntu.edu.tw
Authors	Lin, Chuan-Chi  (A)   Kao, Ching-Huei  (A)
Affiliations:	(A): Department of Agronomy, National Taiwan University

The changes in ionically bound peroxidase activity in roots of NaCl-stressed rice seedlings and their correlation with root growth were investigated. Increasing concentrations of NaCl from 50 to 150 mM progressively decreases root growth. The reduction of root growth by NaCl is closely correlated with the increase in ionically bound peroxidase activity. Since proline and ammonium accumulations are associated with root growth inhibition caused by NaCl, we determined the effects of proline or NH₄Cl on root growth and ionically bound peroxidase activity in roots. External application of proline or NH₄Cl markedly inhibited root growth and increased ionically bound peroxidase activity in roots of rice seedlings in the absence of NaCl. An increase in ionically bound peroxidase activity in roots preceded inhibition of root growth caused by NaCl, NH₄Cl or proline. Mannitol inhibited root growth, but decreased rather than increased ionically bound peroxidase activity at the concentration iso-osmotic with NaCl. The inhibition of root growth and the increase in ionically bound peroxidase activity in roots by NaCl is reversible and is associated with ionic rather than osmotic component.