Poster: Global Change
Abs #
61: Genomic Structure of the Cucumber CPD Photolyase Gene
|
|
Presenter: |
Ioki, Motohide , ss27179@mail.ecc.u-tokyo.ac.jp |
Authors | Ioki, Motohide (A) (B) Nakajima, Nobuyoshi (B) Tamaoki, Masanori (B) Takahashi, Shinya (A) (B) Kondo, Noriaki (A) | | Affiliations: |
(A): Graduate School of Science, The University of Tokyo
(B): National Institute for Environmental Studies
|
|
|
Ultraviolet light with short wavelengths (UVB) causes DNA lesions, retarding the growth and development of plants. Most of the DNA lesions due to UVB are cyclobutane pyrimidine dimers (CPDs). It has been previously shown, through expression analysis of the cucumber CPD photolyase gene (CsPHR), that light-dependent transcriptional activation of CsPHR imparts UVB tolerance to the plant. Further investigation revealed that the transcription of CsPHR is activated most remarkably by light with wavelengths around 300 nm (UVB), indicating an unidentified photoperception mechanism that specifically senses UVB is involved. Studies on the genomic structure, especially the 5f-regulatory region, of CsPHR were urgent for the elucidation of mechanisms through which cucumber plants tolerate and sense UVB. On such supposition, an 8kbp genomic DNA fragment containing the promoter and the coding region of CsPHR consisting of 9 exons and 8 introns was isolated, and the characteristics of the genomic and deduced amino acid sequences were studied. Observed in the 5f-flanking region of CsPHR were putative light-responsive cis-acting regulatory elements. Functional analysis of these elements will facilitate our understanding of the UVB signal transduction and how plants adapt to their radiational environment.
