Poster: Integrated Plant Biology
Abs #
94: In vitro screening for anticancer activity of flavanoid-rich extracts derived from the mature roots of Scutellaria baicalensis on human malignant brain tumor cells
|
|
Presenter: |
Perry, Krya B, krya.wallace@asu.edu |
Authors | Perry, Krya B (A) (B) Scheck, Adrienne C (B) Clark, Dennis (A) Hank, Nicole C (B) | | Affiliations: |
(A): Arizona State University
(B): Barrow Neurological Institute
|
|
|
Flavanoid-rich extracts from mature roots of Scutellaria baicalensis, a herbaceous member of the mint family, have been shown to exhibit anti-proliferative effects on various cancer cell lines. In this study, aqueous (40% ethanol) extracts from S. baicalensis were tested for the ability to inhibit the proliferation of malignant brain tumor cells. Cell lines derived from primary and recurrent brain tumors from the same patient, and cells selected for resistance to the chemotherapeutic agents 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU) and Temozolomide, were used to identify anti-proliferative effects of this extract when used alone and in conjunction with current chemotherapeutic agents. The Alamar BlueTM assay was used to determine the effects of the extract on cellular metabolic activity. Colony forming assays were also used to evaluate whether the metabolically active glioma cells were capable of growth following treatment with 50-200 mg/ml of extract. Primary, recurrent and chemotherapeutic drug resistant cell lines from 3 patients were studied and in each cell line inhibition of cellular proliferation increased markedly with increasing concentrations of extract. Cells selected for resistance to chemotherapeutic agents exhibited an increased sensitivity to the extract. Microscopic examination of the cells further revealed cytotoxic effects 24 hours after treatment. These results demonstrate that a crude extract of S. baicalensis exhibits anti-proliferative activities against cells from primary and recurrent gliomas, as well as gliomas selected for drug resistance. This study supports the notion of using S. baicalensis as an adjuvant treatment to currently used chemotherapeutic agents in an effort to increase therapeutic efficacy without increasing toxicity.
