Poster: Oxidative Stress

Abs # 149: The response to a various stresses in transgenic tobacco plants expressing the antisense genes of biosynthesis and signaling for ethylene

Presenter:	Park, Ky Young , plpm@sunchon.ac.kr
Authors	Park, Ky Young  (A)   Wi, Su Jin  (A)
Affiliations:	(A): Department of Biology, Sunchon National University, Sunchon, Chonnam 540-742, South Korea

Tolerance to a wide variety of environmental stress conditions has been correlated with increased level of antioxidative properties. We have investigated the tolerance against abiotic and biotic stresses by determining the gene expression of antioxidant enzymes using transgenic tobacco plant, in which cellular contents of polyamines were increased by anti-expression of ethylene-biosynthesis genes, 1-aminocyclopropane-1-carboxylic acid (ACC) synthase and ACC oxidase. Transcript levels for antioxidant enzymes, glutathione-S-transferase (GST), ascorbate peroxides (APX), catalase (CAT) and manganase-superoxide dismutase (Mn-SOD), were induced more significantly in transgenic plants than wild-type plant after treatment with abiotic and biotic stresses. We also determined the GUS expression in response to various stresses using transgenic tobacco plants with GUS::ACC synthase promoter. Also we produced transgenic tobacco plants of ethylene-insensitive 3 (EIN 3), which is a transcription factor for ethylene signaling, in sense and antisense orientation. Northern blot analysis and phenotypic assay will be performed in these transgenic tobacco plants after treatment with abiotic and biotic stresses.