Poster: Temperature Responses

Abs # 205: The Barley Transcriptome under Cold Stress: Identification of Differentially Expressed Genes between Winter and Spring Barley.

Presenter:	Svensson, Jan T., jansve@citrus.ucr.edu
Authors	Svensson, Jan T. (A)   Rodriguez, Edmundo  (A)   Wannamaker, Steve  (A)   Close, Timothy J. (A)
Affiliations:	(A): University of California at Riverside
Web Site:	http://www.plantbiology.ucr.edu/people/faculty/close.html

A barley GeneChip was recently made by Affymetrix, enabling us to monitor the expression profile of ca 22,500 barley genes in a single experiment. We are using this array to monitor cold responsive genes. Many plants can increase their freezing tolerance in response to low non-freezing temperatures in a process termed cold acclimation. We subjected a winter and a spring variety (Dicktoo and Morex, respectively) to a controlled low temperature treatment. This enabled us to compare the trancriptome between a more hardy (Dicktoo) and a less hardy (Morex) variety. The experiment was designed for study of the transcriptome under cold-acclimation, cold-hardening and de-acclimation. Our objective is to identify differentially regulated genes that are involved in acclimation and tolerance to freezing.