Poster: Secondary Metabolism

Abs # 291: Inhibition activity of human tumor cell proliferation by Sugar cane vinegar

Presenter:	Kurata, Rie A, rkurata@affrc.go.jp
Authors	Kurata, Rie A (A)   Adachi, Masaru  (B)   Hou, De-Xing  (C)   Fujii, Makoto  (C)   Sugimoto, Akira  (A)   Yoshimoto, Makoto  (A)
Affiliations:	(A): National Agriculture reserch center for Kyushu Okinawa region Upland Farming research Lab.of Upland Crop Utilization (B): Miyakonojo National College of Technology (MNCT) Dept. of Chemistry & Science, (C): Fac. of Agric. Kagoshima University

The profitability is not improved on the sugar cane which is the basic crop of Okinawa and Southwest Islands in Japan, and area under cultivation also decreases year by year. Therefore, multi-use development of the sugar cane is a problem of the crisis from the viewpoint of effective utilization and environmental loading reduction of resources. In until now research, various physiological function-nesses contributed for adult disease and prevention from getting older have been proven by in vitro level in the sugar cane processed goods (brown sugar lump, vinegar). Each component of the sugar cane vinegar adsorbed in the AMBERLITE XAD-2000 column chromatography, and it was divided using the solvent 20,40,60,80,100% methanol solution of the water-methanol system. To investigate their anti-cancer effect,�@induction of apoptosis was tested in human promyelocytic leukemia cells HL-60. The radical scavenging activity was measured by the DPPH method. The condition of DNA of the cell which caused the cell death was confirmed by the fragmentation of DNA. In order to examine the mechanism of the cell death well-informed, it dealt with the 100% methanol fraction, and in the cell after the 48 hour culture, DAPI dyeing and Calcein-AM dyeing were carried out, it was distinguished. Further the 100% methanol fraction was divided by the Folch method, and it was investigated by the effect for the cell proliferation. From present investigation, it was able to be indicated that HL-60 cell death induced by the 100% methanol fraction was the apoptosis.