Poster: Photosynthesis
Abs #
364: De-epoxidation state of lutein and violaxanthin in the seedlings of salt sensitive and salt tolerant plant grown under NaCl salt stress.
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Presenter: |
Misra, Amarendra Narayan , misra@k.u-tokyo.ac.jp |
Authors | Misra, Amarendra Narayan (A) Latowski, Dariusz (B) Strzalka, Kazimierz (B) | | Affiliations: |
(A): Dept. of Botany, Utkal Unversity
(B): IMBB, Jagiellonian University
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The salt susceptible plant, broad bean (Phaseolus vulgaris L. cv. Wonderful John f. tall) and the salt tolerant plant, cabbage (Brassica oleracea L. cv. Capitata f. alba) seedlings were grown in hydroponic cultures supplied with Hoagland nutrient solution. Seven-day-old seedlings were treated with 0.0M, 0.1M, 0.2M and 0.3M aqueous NaCl solutions for 3 days. NaCl treatment induced significant decrease in Chlorophyll content, increase in NPQ, anthraxanthin and zeaxanthin level in Phaseolus seedlings. On the contrary, NPQ decreased, and violaxanthin and lutein levels increased in salt stressed Brassica seedlings. NaCl salt stress induced a significantly faster de-epoxidation of violaxanthin in Phaseolus compared to that of Brassica under growth light (125 mmole m-2 s-1). Similar trend was observed for the de-epoxidation state of lutein. When the leaves were illuminated with saturating light (2000 mmole m-2 s-1, 1h), the de-epoxidation state of violaxanthin increased significantly in NaCl treated Phaseolus over the control leaves but decreased in salt stressed Brassica seedlings. The lutein de-epoxidation state under saturating light condition was similar for Brassica seedlings but there was no significant change in Phaseolus seedlings under NaCl salt stress. The epoxidation state of xanthophyll pigments and NPQ level under saturating light conditions suggests a heat dissipation role of xanthophyll cycle, even in salt stressed seedlings. However, the de-epoxidation state under growth light (low light) condition suggests an inherent genetic difference between salt susceptible and tolerant plants under NaCl salt stress.
