Poster: Nutrient Biology
Abs #
399: Agrobacterium-Mediated Genetic Transformation with a g-TMT Gene to Improve Tocopherol Composition in Soybean
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Presenter: |
Kim, Young-Jin , yjikim@rda.go.kr | Authors | Kim, Young-Jin (A) Yun, Song-Joong (B) Seo, Hong-Yul (A) Park, Tae-Il (A) Kim, Hyun-Soon (A) Lee, Young-Tae (C) Park, Ho-Ki (A) Lee, Moon-Hee (A) | | Affiliations: |
(A): Nat'l Honam Agricultural Experiment Station, RDA
(B): Faculty of Biol. Resources Science, Chonbuk Nat'l University
(C): Research Management Bureau, Rural Development Administration
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Improvement of tocopherol (vitamin E) composition in soybean seeds was attempted through the overexpression of gamma-tocopherol methyltransferase (g-TMT). Soybean cotyledons capable of producing somatic embryos were transformed with Arabidopsis g-TMT gene and somatic embryos were induced on MS medium supplemented with 40 งท/งค 2,4-D. Nineteen regenerated plantlets were obtained on the selection medium from 1,460 cotyledons. Nine putative transformants were confirmed for the stable integration of the transgene into the soybean genomic DNA by PCR and Southern blot analyses, and for the expression of the transgene by HPLC analysis. The a-tocopherol to g-tocopherol ratio was higher in AT-1 and AT-4 transgenic lines than in non-transgenic plants and other T0 lines. The content of a-tocopherol in T0 seeds of AT-1 line was approximately 4-fold higher than that of non-transgenic plants. The result indicates the expression of g-TMT transgene and conversion of a part of the g-tocopherol pool to a-tocopherol by g-TMT.
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