Poster: Vegetative Development

Abs # 414: cDNA microarray analysis on peach fruit

Presenter:	Imai, Tsuyoshi , imagic@affrc.go.jp
Authors	Imai, Tsuyoshi  (A)   Yamamoto, Toshiya  (A)   Matsuta, Nagao  (A)   Yazaki, Junshi  (B)   Ishikawa, Masahiro  (B)   Fujii, Fumiko  (B)   Shimbo, Kanako  (C)   Shimatani, Zempei  (C)   Nagata, Yuko  (C)   Hashimoto, Akiko  (C)   Ohta, Tomoya  (B)   Sato, Yuki  (B)   Honda, Sachiko  (B)   Yamamoto, Kimiko  (C)   Sakata, Katsumi  (B)   Sasaki, Takuji  (B)   Kishimoto, Naoki  (B)   Kikuchi, Shoshi  (B)   Hayashi, Tateki  (A)
Affiliations:	(A): National Institute of Fruit Tree Science (B): National Institute of Agrobiological Sciences (C): STAFF-Institute
Web Site:	http://www.fruit.affrc.go.jp/index-e.html

Two cDNA libraries were constructed from peach fruit: young / suitable for harvest. A cDNA microarray was made using about 1,500 clones randomly picked up. Target mRNAs were purified from fruits of 10 d, 1, 2.5 and 4 months after blooming as well as leaves just after flowering time. Using these system, about one tenth of cDNA clones varying expression levels more than 4-fold were studied. i) Down regulating: polyphenol oxidase etc., ii) up regulating: antimicrobial peptide homologue etc., iii) weak in mid stage: a transcription factor. A cDNA clone belonging to group i), PF10056 encodes a protein having four times of 28 amino acids repeat motif. Although its function is not clear, expression level was very high until 2.5 months.