Poster: Vegetative Development
Abs #
425: Molecular cloning and functional analysis of two E3 RING finger genes from Arabidopsis thaliana
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Presenter: |
Yang, Chang-Hsien , chyang@dragon.nchu.edu.tw |
Authors | Yang, Chang-Hsien (A) Liu, Hsin-Ching (A) Kong, Lih-Ren (A) | | Affiliations: |
(A): Graduate Institute of Biotechnology, National Chung Hsing University, Taichung, Taiwan
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E3 RING finger genes played key roles in the ubiquitin-mediated proteolysis which has a central role in many processes of plant development. E3 RING finger proteins contained a conserved RING motif specifically bound to E2 protein. To investigate the function of E3 RING finger genes, AtRING1 and AtRING2 were isolated and characterized from Arabidopsis. AtRING1 encodes a 181 amino acid protein whereas AtRING2 encodes a 158 amino acid protein. Both AtRING1 and AtRING2 mRNA are expressed throughout the development. However, AtRING1 mRNA is increased during late developmental stage. After flowering, AtRING1 mRNA was expressed higher in flowers and siliques than in leaves and shoot. By contrast, AtRING2 mRNA was expressed higher in shoot than in other organs. Transgenic Arabidopsis plants ectopically expressed AtRING1 or AtRING2 were phenotypically indistinguishable from wild-type plants. By contrast, shoot apical meristem formation was severe altered in Arabidopsis plants ectopically expressed antisense of AtRING1 or AtRING2. The inflorescence and flowers were either not produced or generated abnormally in these antisense transgenic plants. In addition, the leaf morphology was also altered. This result indicated that AtRING1 and AtRING2 should involve in the regulation of shoot apical meristematic activity in Arabidopsis. To investigate AtRING1, 2 action, yeast two-hybrid assays using AtRING1 or AtRING2 as a bait was carried out. Proteins that interacted with either AtRING1 or AtRING2 protein were identified. Further analysis of these genes should lead a deeper understanding of the function for E3 RING finger genes in the ubiquitination pathway in regulating plant development.
