Poster: Vegetative Development

Abs # 438: Expression analysis of Arabidopsis ANL2 gene

Presenter:	Kubo, Hiroyoshi , hkubo77@gipac.shinshu-u.ac.jp
Authors	Kubo, Hiroyoshi  (A)   Kishi, Mutsumi  (A)
Affiliations:	(A): Department of Biology, Faculty of Science, Shinshu University

ANTHOCYANINLESS2 (ANL2) gene encodes a homeodomain protein that belongs to the HD-GL2 family. ANL2 gene is responsible for the accumulation of anthocyanin in aerial parts and the cellular organization of the primary root. In anl2 mutant, the accumulation of anthocyanin in subepidermal tissues is reduced. In addition, extra cells are formed between epidermal and cortical layers of the primary root. To examine the functin of ANL2 gene, we examined expression pattern of ANL2 gene by using GUS gene as a reporter. A 2kb-promoter::GUS construct alone did not induce GUS expression effectively and a downstream sequence is required in addition to the promoter. The expression pattern of GUS almost coincided with the previous indication obtained from the mutant phenotype. In aerial parts, apical region, young leaves, young axillary bud and flower showed GUS activity. In floral organs, stamens and pistil showed GUS activity but petals and sepals did not. Root tip showed strong GUS activity. All tissues of the root tip showed GUS activity but endodermis had much stronger activity than the other root tissues. This expression pattern in the primary root had not been expected because mutant phenotype is observed not in endodermis but epidermis/cortex. The response of gene expression to various environmental factors is being investigated using the GUS transgenic lines.