Poster: Reproductive Development
Abs #
462: Identification of anther specific genes by using rice cDNA microarray
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Presenter: |
Endo, Makoto , Makoto@pb-unix.agr.iwate-u.ac.jp | Authors | Endo, Makoto (A) (B) Tsuchiya, Tohru (C) Matsubara, Hitoshi (A) (F) Masuko, Hiromi (A) Hakozaki, Hirokazu (A) Fikuda, Hiroo (D) Demura, Taku (E) Watanabe, Masao (A) | | Affiliations: |
(A): Faculty of Agriculture, Iwate university
(B): Graduate School of Life Sciences, Tohoku university
(C): Faculty of Bioresources, Mie University
(D): Department of Biological Sciences, Graduate School of Science, The University of Tokyo
(E): Plant Science Center, The Institute of Physical and Chemical Research
(F): Adachi Higashi High School
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The developmental programs of male reproductive organs are regulated by the complicated genetic pathway. In order to identify the genes, which express specifically during anther development, we screened 4,304 cDNA clones isolated from anther or pistil cDNA library of rice.
To monitor the gene expression patterns during development of sexual reproductive organs, we isolated anther and pistil tissues at different developmental stages according to the number of the nuclei/cells in the male gametophytes; uni-cellular microspore stage (stage 1), bi-cellular pollen stage (stage 2) and tri-cellular mature pollen stage (stage 3). mRNA derived from anther or pistil tissues in different developmental stages was labeled with Cy5. In contrast, mRNA from leaf tissues, a vegetative organ, was labeled with Cy3. The samples were labeled with different dyes, then mixed and hybridized simultaneously to a cDNA microarray.
Cluster analysis of the microarray data revealed 162 independent cDNA groups, which were specifically expressed in anthers. Out of these 162 cDNA, 91 cDNA clones were specifically expressed in stage 3 anther tissues, 31 cDNA clones were specifically expressed in stage 2 anther tissues, 20 cDNA clones specifically expressed in stage 1 anther tissues and 20 cDNA clones were specifically expressed during stage 1 to stage 2 anther tissues.
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