Poster: Photomorphogenesis

Abs # 499: NDPKs of Brassica campestris have Different Regulation Functions in Seed Germination of Arabidopsis thaliana

Presenter:	Shin, Dong Ho, sdhsdong@hanmail.net
Authors	Shin, Dong Ho (A)   In, Jun Gyo (A)   Lim, Young Pyo (B)   Hasunuma, Kohji  (C)   Choi, Kwan Sam (A)
Affiliations:	(A): Department of Applied Biology & Chemistry, Colleage of Agriculture & Life Sciences, Chungnam National University (B): Department of Horticulture, Colleage of Agriculture & Life Sciences, Chungnam National University (C): Yokohama City University, Kihara Institute for Biological Research

Recently, there are reported that NDPK genes have the significant functions in light signal transduction pathway of the plant. We have cloned and characterized three NDPK cDNAs (BcNDK1, BcNDK2 and BcNDK3) from Chinese cabbage (Brassica campestris) by using the cDNA library screening and RT-PCR. From phylogenic tree analysis, All BcNDKs show high homology to other plant NDPK genes, especially more 80% with those of Arabidopsis (AtNDK1, 2 and 3), respectively. These genes also were expressed differently on the development stage such as germination, seedling development, tissues and flower organs. To know the function of these genes on the growth and development of plant, we have selected several mutants from Arabidopssis which are increased or reduced the expression of these genes by gene transformation, respectively. By mutant analysis, we confirmed that NDPK 1 and NDPK2 have regulation functions in seed germination by the different light treatment