Poster: Hormones

Abs # 563: The role of hormones in regulating plant wound-stress responses

Presenter:	David, Lisa I, lidavid@unity.ncsu.edu
Authors	David, Lisa I (A)   Davies, Eric  (A)
Affiliations:	(A): North Carolina State University

Wounding stress is a continuous threat to the survival of all organisms and in crop plants it leads to a dramatic reduction in crop yield. The signaling pathways that allow plants to respond to wounding stress are known to be complex. Many plant hormones such as jasmonic acid (JA), salicylic acid (SA), and ethylene have been identified as critical factors for the induction of wound-responsive genes in a number of plant species. However, the specific roles of each hormone in vivo and how different hormones interact in the wound-stress response are largely unknown. Elucidation of the specific roles of these plant hormones and their interactions with one another in response to wound stress is a major focus of our wound research. The major goal of this research is to utilize Arabidopsis JA, SA, and ethylene biosynthetic and signaling single and double mutants to analyze the role of each hormone separately and together in wound-responsive gene induction. Arabidopsis wild type, ein2, npr1 and jar1 plants were wounded for different times, RNA extracted, electrophoresed, blotted and probed for several putative wound-upregulated transcripts, including bZIP, MPK4, LOX2, WAK1, VSP1, TCH3, PR1, and THI2.1 The expression data will be presented and discussed.