Poster: Plant Pathogen/Symbiont Interactions

Abs # 712: Probenazole-induced protein (PBZ) is involved in plant cell death

Presenter:	Kang, Kyu Young , kykang@nongae.gsnu.ac.kr
Authors	Kang, Kyu Young  (A) (B)  Kim, Sun Tae  (A) (B)  Yu, Seok  (A)   Kim, Sang Gon  (A) (B)  Kang, Sun Young  (A)
Affiliations:	(A): Division of Applied Life Sciences, (B): Plant Molecular Biology and Biotechnology Research Center

Cell death plays important roles in the defense and development of plants as in other multicellular organisms. The expression of a probenazole-induced protein(PBZ) coincided with several developmental events associated with programmed cell death (PCD) occurred in such as leaf senescense, root aerenchyma formation, cleoptile, root cap, seed aleurone layer, and hypersensitive-response. The expression levels and localizations of PBZ in tissue of PCD were identified by Western blot and immunohistochemistry analysis. Purified recombinant PBZ was induced hypersensitive cell death in rice suspension cells and tobacco leaves in dosage dependent manner. Pretreatment of antibody raised against PBZ blocked cell death caused by PBZ. These results demonstrated that PBZ was involved in plant cell death