Poster: Plant Pathogen/Symbiont Interactions

Abs # 730: Bean yellow dwarf virus (BeYDV) V1 movement protein targets endoplasmic reticulum-derived vesicles

Presenter:	Huang, Zhong , zhong.huang@asu.edu
Authors	Huang, Zhong  (A)   Mason, Hugh S (A)
Affiliations:	(A): Arizona State University

The V1 protein of bean yellow dwarf virus (BeYDV) is required for cell-to-cell and long distance movement of the virus. To determine its localization and association with host cellular structures, the V1 protein was expressed as a fusion to yellow fluorescent protein (YFP) in plant cells by different transient expression methods. In bombarded tobacco NT-1 cells, V1:YFP accumulated in perinuclear “ring”, in association with cytoplasmic strands and in “disc-like”, vesicular compartments. In transfected N. benthamiana mesophyll protoplasts, V1:YFP distributed throughout the cytoplasm and at fluorescent vesicles. Using V1 deletion constructs, a central hydrophobic region within the V1 was found to be responsible for vesicle formation. Co-transfection experiments with an ER marker CFP:ER demonstrate that the V1 co-localizes with the ER on the vesicles and solely contributes to vesicle formation. Additionally, subcellular fractionation and biochemical treatment of protein extracts reveals that the V1 behaves as an integral membrane protein. The possible role of the association of V1 protein with the ER and the ER-derived vesicle in BeYDV intracellular trafficking is discussed.