Poster: Intercellular Signaling
Abs #
807: Analysis of gene expression in response to nitric oxide in maize seedlings using cDNA microarrays
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Presenter: |
Madasamy, Parani , mparani@utnet.utoledo.edu |
Authors | Madasamy, Parani (A) Rudrabhatla, Sairam (A) Weirich, Heatherbea (A) Rangasamy, Elumalai (B) Galbraith, David W. (B) Goldman, Stephen L. (A) | | Affiliations: |
(A): University of Toledo, Plant Science Rsearch Center
(B): University of Arizona, Department of Plant Science
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Nitric oxide (NO) is an important signal molecule for the activation of muscle relaxation, inflammatory response, innate immune response and programmed cell death in animals. It seemed that NO and several key components involved on NO-mediated signaling might be operative in plants too. It was subsequently shown that NO is in fact ubiquitous in plants. Occurrence of the second messengers of NO, cGMP and cADPR in plants has also been demonstrated. However, the gene for nitric oxide synthase (NOS), which is a key enzyme in NO production in animals, has not yet been cloned from plants. Exogenously supplied NO in plants has much diverse effects on plant growth and metabolism that it is associated with seed germination, growth, differentiation, maturation, senescence and plant defense mechanisms against biotic and abiotic stresses. Hence, it seems to be in parallel as well as anti-parallel with other signaling pathways of gibberellic acid, jasmonic acid, ethylene, hydrogen peroxide etc. In order to understand the effect of NO on gene expression, we are studying the effect of exogenously supplied NO on maize seedlings using cDNA microarrays. Three-week-old seedlings of maize (B73) were supplied with 200µM of sodium nitroprusside (SNP) for 3 hours and then supplied with 100µM of methylene blue for 3 hours. While SNP is a donor of NO, methylene blue inhibits NO production and or action. Seedlings in Hoagland’s solution were used as control. Total RNA was isolated from leaf tissues and purified mRNA was used for labelling with flourescent dyes and hybridization to maize unigene microarrays. Changes in gene expression profile in response to NO treatment is discussed.
