Poster: Emerging Technologies
Abs #
894: Metabolomic Analysis of Cold Acclimation in Arabidopsis thaliana
Metabolomics is the study of the set of small molecules or metabolome, in a given tissue or organism. Non-targeted analysis of metabolites gives a direct insight into the actual components of a phenotype, which is a step beyond information generated by genomics and proteomics. Fourier Transform Ion Cyclotron Mass Spectrometry has the highest mass resolution and accuracy to allow positive identification of metabolites, based on their masses. This unbiased approach was used to study the changes in metabolite levels in leaf samples of Arabidopsis thaliana plants grown under varying conditions of cold acclimation, deacclimation, and cold reacclimation. This led to the detection of over 1600 masses that are representative of at least as many metabolites. Intensities of metabolites from samples grown under different cold acclimation conditions were compared, and yielded both expected and novel metabolite changes. The results of these studies will be presented.
