Poster: Biotech Risk Assessment

Abs # 899: Qualitative PCR analysis for detection of genetically modified lines of soybean, maize, and potato

Presenter:	Lee, Theresa , tessyl1@rda.go.kr
Authors	Lee, Theresa  (A)   Rho, Jae-Hyun  (A)   Ahn, Il-Pyung  (A)   Kang, Sang-Ho  (A)   Kim, Tae-San  (A)   Park, Yong-Hwan  (A)   Kim, Young-Mi  (A)
Affiliations:	(A): National Institute of Agricultural Biotechnology

To cope with labeling regulation on genetically modified (GM) organisms in Korea, several sets of primers have been developed to detect foreign genes specifically in imported soybeans, maize, and potatoes. Target crops include Roundup Ready soybean (RRS), 5 different maize lines including Mon810, GA21, Event176, Bt11, and T25, and 3 potato lines including NewLeaf, NewLeaf-Y, and NewLeaf-Plus. Specificity of primer sets was confirmed by PCR with line-specific primers individually. Sensitivity for RRS was revealed as 0.5% by performing duplex PCR with transgene- and endogenous single copy gene-specific primers. Five lines of GM maize and 3 lines of GM potato were subjected to multiplex PCR respectively. This multiplex PCR method was useful to detect each and every transgene in the mixed lines tested.