Poster: Comparative Genomics

Abs # 922: Comparative structural cytogenetic analysis 1 Mb surrounding the FLC locus in the crucifers

Presenter:	Jackson, Scott A, sjackson@purdue.edu
Authors	Jackson, Scott A (A)   Sanyal, Abihjit  (A)
Affiliations:	(A): Purdue University

A 1Mb BAC/PAC contig surrounding the FLC locus in Arabidopsis was selected to structurally analyze this region in related crucifer plants. The Arabidopsis BACs/PACs have been used as probes for fluorescence in situ hybridization on extended DNA fibers from related crucifers (A. arenosa, A. neglecta, A. lyrata, A. halleri, Cochlearia semperivium, Thalaspi oxycera, T. rolulare, T. caerulescens, Bornmeullera tymphaea, Peltaria emerginata ). Mapping on extended DNA fibers is essentially mapping at the Watson-Crick resolution, 1 micrometer of physical distance is equivalent to ~2,800 bp of DNA. There is sufficient conservation of DNA sequence and order that Arabidopsis BACs/PACs can be hybridized across species. We will present data on both long-range and micro changes in the genomic structure of this region using a high-resolution, molecular cytogenetic approach.