Poster: Genomics Resources

Abs # 930: Making BAC libraries from algae, non-seed and seed plants: fundamental public resources for plant science

Presenter:	Mandoli, Dina F., mandoli@u.washington.edu
Authors	Mandoli, Dina F. (A)   Banks, Jody  (B)   dePamphilis, Claude  (C)   Carlson, John  (C)   Tomkins, Jeff  (D)   Kudrna, David  (E)   Luo, Meizhong  (E)   Wing, Rod A (E)   Olmstead, Richard  (A)
Affiliations:	(A): University of Washington (B): Purdue University (C): Pennsylvania State University (D): Clemson University (E): University of Arizona
Web Site:	http://faculty.washington.edu/mandoli

The evolutionary transition to life on land and the diversification that followed required solving a series of problems such exposure to air and solar irradiation, life in a desiccating environment, etc. Plants solved these challenges with biochemical, cellular, anatomical and morphological innovations. We will provide access to the genetic basis of most of these innovations by making high-quality bacterial artificial chromosome, or BAC, libraries of 16 green plant species which are key in the evolutionary transition to land. These taxa include: Volvox carteri, Caulerpa mexicana, Mesostigma viride, Coleochaete orbicularis, Chara sp., Marchantia polymorpha, Anthoceros sp., Lycopodium lucidulum, Angiopteris evecta, Ceratopteris richardii, Marsilea quadrifolia, Amborella tricopoda, Nuphar adventa, Acorus gramineus, Liriodendron tulipfera, Mimulus guttatus. All libraries will be made available to the public for a minimal cost via Clemson University Genome Institute and Arizona Genomics Institute as soon as they are made and characterized. Key innovations and our current progress, including the timing of the planned availability of these 16 BAC libraries to the public at large, will be presented. Funded by the NSF's First One Hundred Program, 2002-2005, IBN #0208502 (lead institution), #020211611, #0207110, and #0207202.