Poster: Genomics Resources
Abs #
937: Generation of expression sequence tags from full-length, normalized and size-selected cDNA libraries of the Japanese morning glory
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Presenter: |
Hoshino, Atsushi , hoshino@nibb.ac.jp |
Authors | Hoshino, Atsushi (A) Seki, Motoaki (B) Shin-I, Tadasu (C) Nishide, Hiroyo (A) Carninci, Piero (B) Kamiya, Asako (B) Shiraki, Toshiyuki (B) Nitasaka, Eiji (D) Uchiyama, Ikuo (A) Shinozaki, Kazuo (B) Hayashizaki, Yoshihide (B) Yuji, Kohara (C) Iida, Shigeru (A) | | Affiliations: |
(A): National Institute for Basic Biology
(B): RIKEN Genomic Sciences Center
(C): National Institute for Genetics
(D): Kyusyu University
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The Japanese morning glory (Ipomoea nil) is a traditional horticultural plant in Japan. A number of spontaneous mutants have been isolated, and more than 200 genetic loci have been reported. Most of these loci are related to the colors and shapes of its flowers and leaves. The wild type of the plant blooms light blue flowers accumulating an acylated peonidin glycoside named Heavenly blue anthocyanin (HBA) in their vacuole. Both the HBA production and alkalization of the vacuolar pH are key determinants for blue flower coloration. To aid the identification of the genes for the coloration and the shape determination of the flowers, we performed a large-scale expression sequence tags (ESTs) analysis.
The cDNA libraries were generated from mRNA isolated from mixture of opened flowers and flower buds. To select full-length cDNA clones, we prepared a cDNA library using biotylated cap trapper method based on chemical introduction of a biotin group into the diol residue of the CAP structure. Normalized and size-selected cDNA libraries were also prepared in order to avoid biased representation of cDNA clones. Total 56,000 ESTs were established, and 12,939 (5'-end) and 12,960 (3'-end) ESTs containing approximately 5,500 non-redundant full-length clones were deposited in GenBank/EMBL/DDBJ with accession numbers BJ553078-BJ578976. These ESTs contain various candidate genes for flower coloration and morphogenesis. Our cDNA clones and ESTs would be powerful sources for functional analyses of the genes involving in the biology of flowers in the plant.
