Poster: Genomics Resources

Abs # 946: Analysis of expressed sequence tags from root nodule of Glycine max

Presenter:	Lee, Hyoungseok , soulaid@freechal.com
Authors	Lee, Hyoungseok  (A)   Hur, Cheol-Goo  (B)   Oh, Chang Jae  (A)   An, Chung Sun  (A)
Affiliations:	(A): Seoul National University (B): National Center for Genome Information, Korea Research Institute of Bioscience and Biotechnology (KRIBB)

For the high throughput screening of useful genes, three cDNA libraries specific for the root nodule of soybean were constructed from three different stages of root nodules after inoculation with Bradyrhizobium japonicum USDA110. The nodule-specific EST database was constructed from these cDNA libraries. To eliminate redundancy, we performed the differential hybridization using high redundant clones as probes. As a result, 5,469 cDNA clones were sequenced, these EST sequences were clustered into 2,511 non-redundant sequences comprised of 769 non-redundant clusters and 1,742 singletons. Similarity searches against public non-redundant protein database, soybean EST database and MIPS database search were done. From these results we could make the functional catalog of our ESTs, including 1) several nodule specific genes named nodulin, 2) various stress-responsive genes, 3) genes related to carbon and nitrogen metabolism, 4) putative kinase and phosphatase. cDNA clones and EST database obtained from this study will be used for cDNA microarray to study root nodule development and of plant development.