Poster: Genomics Resources
Abs #
956: Profiling growth-phase dependent gene expression of tobacco BY-2 cells using comprehensive EST microarray
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Presenter: |
Matsuoka, Ken , kenmat@psc.riken.go.jp |
Authors | Matsuoka, Ken (A) Tashiro, Gen (A) Horiguchi, Tatsuya (A) Sasaki, Mami (A) Demura, Taku (A) Fukuda, Hiroo (A) | | Affiliations: |
(A): Plant Science Center, RIKEN
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Tobacco cell line BY-2 is the most commonly used cell line for basic plant research, especially on cytokinesis and protein transport. This cell grows rapidly and can be easily transformed with very high frequency. Unfortunately, the use of BY-2 cells had a disadvantage because little genome or cDNA sequences of tobacco could be found in public database.
We have been analyzing protein transport and protein modification in plant cells using this tobacco cell line as a model. However, the disadvantage described above appeared to prevent a rapid progress of our research. Therefore, in the Morphogenesis Research Group in RIKEN Plant Science Center, we have started TAB ( T ranscriptome A nalysis of B Y-2) project to accumulate the transcription information of tobacco BY-2 cells. At the first phase of this project, we got about 9,000 EST sequences. The library used for this analysis is a normalized cDNA library made from lag, log and stationary phase BY-2 cultures. The 9,000 EST sequences were classified into ca. 7,000 unique clusters. These EST sequence will be already released from DDBJ when this meeting is held.
For the second phase of the project, we set up a system to make high-density microarrays that contain PCR-amplified ESTs. We are conducting microarray analysis using different phases of cultures. Histone genes are highly expressed in log-phase cells as expected. Expressions of some vacole-related transcripts were up regulated in stationary phase cells, probably due to the rapid expansion of cell size at this growth phase. We are currently conducting detailed analysis of the microarray data and the result of the EST and microarray analysis will be discussed in this presentation.
