Poster: Genomics Resources

Abs # 960: Construction and characterization of full-length cDNA libraries from Oryza sativa L. ssp. Nakdong

Presenter:	Nahm, Baek-Hie , kim750@ggbio.com
Authors	Nahm, Baek-Hie  (A)   Kim, Jeong-Sook  (A)   Jun, Kyong-mi  (A)   Chung, Pil-Joong  (A)   Lee, Tae-Ho  (A)   Kim, Min-Jeong  (A)   Song, Sang-Ik  (A)   Kim, Ju-Kon  (A)   Kim, Yeon-Ki  (A)   Baek-Hie, Nahm  (A)
Affiliations:	(A): Genomics and Genetics Institute, GreenGene Biotech, Myongji University

The whole genome of rice, one of the most important staple cereals feeding a thirds of whole human population on earth, has been fully sequenced by several research groups because of its economic and strategic importance. Future researches will put foci on gaining insights to have finer genome structure and biological information of those genes. We launched a rice full-length cDNA project aiming multiple purposes such as accumulating databases of open reading frames of rice genes, manufacturing cDNA microarray chips to investigate gene expression in genome scale, and finally supporting the whole rice research communities. Nine cDNA libraries were constructed using mRNA prepared from tissues of Oryza sativa L. ssp. Nakdong and its transgenic lines on in combination of various developmental stages and stress treatments. A combined method of oligo-capping and PCR to capture and amplify full-length cDNA was adopted. In total, 60,000 clones of fourteen libraries from wild and its transgenic type plants, were sequenced in 5' and/or 3' directions. Sequences were analyzed with BLAST and CLUSTALW algorithms. The numbers of total singletons and full-length clones are being analyzed.