Poster: Metabolic Engineering

Abs # 1006: Expression of a cyanobacterial sucrose-phosphate synthase in transgenic rice plants

Presenter:	Lee, Sang-Kyu , kyuri93@cvs2.kyunghee.ac.kr
Authors	Lee, Sang-Kyu  (A)   Shin, Woon-Chul  (A)   Lee, Jang-Wook  (A)   Yoon, Seok-Yoon  (A)   Lee, Soo-Jeong  (A)   Lee, Youn-Hyung  (A)   Bhoo, Seong Hee  (A)   Jeon, Jong-Seong  (A)   Hahn, Tae-Ryong  (A)
Affiliations:	(A): Kyung Hee University

Sucrose-phosphate synthase (SPS) is a key regulatory enzyme in sucrose synthesis. To investigate the role of SPS in carbon partitioning, we produced transgenic rice plants overexpressing a cyanobacterial SPS from Synechocystis sp. PCC 6803. The gene was expressed under the control of the maize Ubi1 promoter in transgenic plants. Southern and Northern blot analyses confirmed the integration and the expression of the transgene in four transgenic rice lines. All of the four transgenic lines analyzed showed abnormal vegetative and reproductive developments. Analysis of primary metabolites revealed that the sucrose contents were significantly higher in transgenic plants than in wild type.