Poster: Transcription Regulation

Abs # 1053: Construction of Ethanol-inducible Expression Vector and Its Expression Analysis in Arabidopsis Protoplast

Presenter:	Hee-Jung, Yuck , treasure@hanmail.net
Authors	Hee-Jung, Yuck  (A)   Sung-Jin, Kim  (A)   Youn-Hee, Choi  (A)   Kwang-Woong, Lee  (A)
Affiliations:	(A): School of Biological Sciences, Seoul National University,

In order to establish ethanol-inducible gene expression system in the plant, alcoholic fermentable gene ADH2 (Alcohol Dehydrogenase 2) promoter of yeast (Saccharomyces cerevisiae) and ADR1 transcription factor were isolated. ADH2 gene is expressed by the transcription factor, ADR1 to use alcohol as carbon source. In this experiment, The transcription factor ADR1 was introduced into pBI121 vector and ADH2 promoter was introduced into pCAMBIA1303 vector. Finally, p35S-ADR1-tnos region of pBI121 vector transferred modified pCAMBIA1303 vector and ethanol-inducible expression vector was constructed. To examine the reaction of ethanol-inducible expression vector in Arabidopsis protoplast, expression level of b-glucuronidase (GUS) gene and Green Fluorescence Protein (GFP) was monitered after 2 % ethanol was treated to transgenic protoplast. All of the three ethanol-inducible plant expression vectors showed the GFP expression after 2% ethanol treatment. Among them, pADH2:p35S fused plant expression vector of those showed the highest GFP expression level. The results suggest that the ethanol-inducible plant expression vector may work under ethanol treatment.