Poster: Late and Moved Abstracts
Abs #
1365: An Investigative Approach to the Health Promoting Properties of the Genus Vaccinium
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Presenter: |
Kraft, Tristan F.B., tkraft@uiuc.edu | Authors | Kraft, Tristan F.B. (A) Rogers, Randy (A) Yousef, Gad (A) Schmidt, Barbara (A) Knight, Christopher T.G. (A) Seigler, David (C) Pezzuto, John M. (B) Lila, Mary Ann (A) | | Affiliations: |
(A): Department of Natural Resources and Environmental Sciences,University of Illinois, Urbana
(B): Department of Medical Chemistry and Pharmacognosy, University of Illinois, Chicago
(C): Department Plant Biology,University of Illinois, Urbana
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Berries in the genus Vaccinium, such as blueberries and cranberries, have compounds that are antimicrobial, reduce membrane lipid peroxidation in vitro, moderate the effects of inflammatory stress, provide urinary tract protection, are cardioprotective, and protect against different stages of carcinogenesis. We have demonstrated that fractions of a 70% acetone extract of V. angustifolium, lowbush blueberry, fruits have activity in a range of anti-cancer bioassays: quinone-reductase (initiation stage, 3.5 ėg/mL to double activity), cyclooxygenase-2 (initiation stage, 77% inhibition), ornthine decarboxylase inhibition (significant inhibition of promotion stage), and a hepatocyte assay (proliferation stage, 94% inhibition). The activity and composition of the fraction active in the cycloxygenase-2 inhibition assay was verified by comparing the NMR spectra and the bioassay results of the fruit fraction to a sample comprised of commercially available components; the composition was found to be catechin:epicatechin, 2.5:1. Cell cultures derived from the related species Vaccinium x pahalae (ohelo) readily accumulate a parallel range of bioactive flavonoid compounds in a consistent manner without interfering compounds, such as pectins and sugars, that otherwise impede fractionation and bioassays from fruit. Therefore, ohelo is an excellent model culture system for comparisons and in depth investigations of parallel bioactive compounds from fruits, which allows for labeling of bioactive compounds so their movement and incorporation in mammalian systems can be tracked. Initial results indicate that ohelo cells incorporate 34.7% of 14C label supplied to production medium within a two week growth period, and flavonoid-rich fractions of ohelo incorporate 13.1% of the total label.
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