Poster: Late and Moved Abstracts

Abs # 1370: Authentication of Scutellaria baicalensis Georgi by the sequence variations of non-coding regions of chloroplast DNA and internal transcribed spacers

Presenter:	Chiou, Shu-Jiau , 771134@itri.org.tw
Authors	Chiou, Shu-Jiau  (A)   Lee, Win-Ching  (A)   Lee, Cherng-Yu  (A)   Tong, Teng-Soung  (A)
Affiliations:	(A): Industrial Technology Research Institute

Scutellaria baicalensis Georgi has long been used as an immunostimulating, fever-relieving and anti-inflammatory agent in the prescription of Chinese traditional medicines. Scutellaria baicalensis is usually sold in the form of dried slices and is difficult to be distinguished from other Scutellaria by morphological and histological characteristics. Our objective was to develop molecular markers by exploring sequence variations of non-coding regions of the cpDNA and the internal transcribed spacers (ITS) of rDNA to distinguish S. baicalensis from other commonly used species, such as S. amoena and S. hypericifolia. Three intergenic regions (IGS) of cpDNA (psbA-trnH, trnS-trnG and trnD-trnY) and ITS of rDNA were PCR amplified and analyzed. No difference was detected in the regions of trnD-trnY and trnS-trnG. In the psbA-trnH region, S. baicalensis and S. amoena had one nucleotide difference within 407 aligned bases; while S. baicalensis differed from S. hypericifolia with three deletions (128, 10 and 24 bases) and three single-base substitutions. The sequences of ITS1 and ITS2 in S. baicalensis shared 81.3% and 74.7% identity with S. amoena, and 82.4% and 76% identity with S. hypericifolia, respectively. Our results suggested that the intraspecific sequences of the non-coding IGS of cpDNA and the ITS of rDNA can be applied in authenticating the Chinese medicinal materials.