Poster: Late and Moved Abstracts

Abs # 1372: Analysis of gene expression during incompatible wheatgrass-BYDV/CYDV and compatible oat-BYDV/CYDV interactions by real-time PCR

Presenter:	Balaji, Boovaraghan , bbalaji@purdue.edu
Authors	Balaji, Boovaraghan  (A)   Anderson, Joseph M (B)
Affiliations:	(A): Dept of Agronomy, Purdue University (B): USDA-ARS, Dept of Agronomy, Purdue University

Barley yellow dwarf virus and Cereal yellow dwarf virus (YDVs) are the most endemic and severe viral diseases in cereal crops causing significant yield losses. Wheatgrass (Thinopyrum intermedium) a wild relative of wheat, however, is very resistant to various isolates of YDV whereas many oat cultivars are highly susceptible. In an effort to isolate genes that may be responsible for resistance in wheatgrass and susceptibility in oats, we used a combination of suppressive subtractive hybridization (SSH) and mirror orientation selection (MOS). The latter procedure was used on the SSH libraries to minimize the rate of false positive clones identified by differential hybridization. SSH and MOS were done on YDV-treated and untreated resistant wheatgrass and susceptible oat followed by cDNA macroarray differential hybridizations. Our results show that 90% of the putative differentially expressed genes selected from the wheatgrass library and 81% from the oat library were confirmed with real-time PCR to be differentially regulated. A few of these genes in YDV-treated wheatgrass showed significant similarity to genes encoding plant defense and stress responses. The level of expression of some of these wheatgrass-derived genes is either suppressed or not different when they were analyzed with un-subtracted libraries of YDV treated and untreated oats. The changes in the gene expression profile between YDV-incompatible wheatgrass and compatible oat are beginning to delineate the significant differences in plant responses during interaction with YDV.