Poster: Late and Moved Abstracts

Abs # 1376: Assessment of utility of meiosis-associated promoters of lily for induction of germinal Ds transposition in transgenic rice

Presenter:	Toriyama, Kinya , torikin@bios.tohoku.ac.jp
Authors	Toriyama, Kinya  (A)   Morita, Ryohei  (A)
Affiliations:	(A): Graduate School of Agricultural Science, Tohoku University

In order to suppress the somatic excision of Ds element and increase the independent transposition events of the Ac/Ds transposon tagging system in rice, we employed promoters of two meiosis-specific genes of lily, LIM10 and LIM18. Analysis of each promoter-GUS fusion in rice demonstrated the meiosis and microspore-specific activation of LIM10 promoter, whereas LIM18 promoter directed GUS expression also in somatic tissues. A fusion between LIM10 or LIM18 promoter and a cDNA for Ac transposase (AcTP) was constructed and introduced into rice plants. They were crossed with plants carrying the Ds element, Ds-GUS T-DNA. Ds element excision was detected in anthers but not in leaves of all the F1 plants containing LIM10::AcTP, although it was detected in leaves with LIM18::AcTP. Independent transposition was demonstrated by Southern blot analysis in F2 plants. �@Both promoters induced independent germinal transposition with the frequency of approximately 1%. �@These results demonstrate that the LIM10 and LIM18 promoters have the potential to induce high frequency of independent germinal transposition. Especially the LIM10 promoter, lacking induction of somatic transposition, is considered to be useful for improving transposon-tagging efficiencies in rice.