Poster: Late and Moved Abstracts

Abs # 1377: Molecular characterization of Rf1, the fertility restorer gene for BT-type CMS rice, that promotes the processing of mitochondrial B-atp6 mRNA.

Presenter:	Kazama, Tomohiko , tomo-k@bios.tohoku.ac.jp
Authors	Kazama, Tomohiko  (A)   Toriyama, Kinya  (A)
Affiliations:	(A): Graduate School of Agricultural Science, Tohoku University

A fertility restorer gene (Rf1) of BT-type cytoplasmic male sterility (CMS) in rice has been reported to be responsible for the processing of mitochondrial mRNA of aberrant atp6 (B-atp6). We have carried out map-based cloning of the Rf1 gene and found that a 4.7-kb genomic fragment of a restorer line promoted the processing of aberrant B-atp6 mRNA when introduced into a CMS line. The genomic fragment contained a single ORF encoding 791 amino acids protein with a mitochondrial targeting presequence of 26 amino acids and 18 repeats of the 35-amino-acid pentatricopeptide repeat (PPR) motif. The cloned PPR-gene is a possible candidate of Rf1. A nonrestoring genotype was identified to have deletions within the coding region.