Poster: Late and Moved Abstracts

Abs # 1404: Mapping and characterization of glassy trichome mutants in Arabidopsis

Presenter:	Brininstool, Ginger M, gbrinin@lsu.edu
Authors	Brininstool, Ginger M (A)   Larkin, John C (A)
Affiliations:	(A): Louisiana State University

On several independent occasions, glassy trichome mutants have been identified in mutagenesis screens of Arabidopsis thaliana. These mutants are termed glassy due to the trichome cell walls being transparent instead of opaque like wild-type trichomes. To date, only CONSTITUTIVE PATHOGEN RESISTANCE5 (CPR5) has been cloned and shown to encode an unknown protein in Arabidopsis. Some characterization of TRICHOME BIREFRINGENCE (TBR) and NOECK (NOK) have been published in the literature, though neither has been cloned. Trichomes on tbr have reduced birefringence and contain less paracrystalline cellulose than wild-type trichomes, thereby implicating TBR in secondary cell wall development. With the characterization of tbr as impetus for characterization of other glassy mutants, presented here is work on cpr5, nok, and a novel glassy mutant deflated (dfl). These glassy mutants have trichomes with reduced birefringence. However, only cpr5 leaves have less paracrystalline cellulose. Identification of the genes disrupted in nok and dfl is in process, with nok mapping to the top of chromosome three and dfl mapping to the top of chromosome 5. Double mutants between these mutants and another trichome mutant, triptychon (try), has revealed an interesting genetic interaction among CPR5, NOK, DFL, and TRY.