Poster: Late and Moved Abstracts
Abs #
1423: Identification and characterization of a heat-shock responsive phosphosulfolactate synthase-like gene from tomato
|
|
Presenter: |
Charng, Yee-yung , yycharng@gate.sinica.edu.tw |
Authors | Charng, Yee-yung (A) Liu, Nai-Yu (A) Ko, Swee-Suak (A) Wang, Chiu-Chung (A) Li, Hsiao-Yuan (A) Yeh, Kuo-Chen (A) | | Affiliations: |
(A): Inst. BioAgric. Sci., Academia Sinica
|
|
|
Suppression subtractive hybridization and DNA microarray techniques were employed to identify novel heat-shock (HS) responsive genes in tomato tissues. About one thousand clones, picked from subtractive libraries prepared from HS-treated tomato root or shoot, were spotted onto glass slides along with 6,000 unique clones from tomato root ESTs obtained from Clemson University Genomics Institute (CUGI). Microarray analysis demonstrated that 130 clones showed greater than 5-fold up-regulation upon HS treatment both in the shoot and root tissues. Most of these up-regulated clones came from the HS subtractive libraries and only two clones were derived from CUGI. Sequencing analysis shown that most of these clones belong to various well-characterized heat shock protein (HSP) gene families. A number of these clones matched to nucleotide sequences annotated as unknown or hypothetical protein in databases, one of which shared high homology to Arabidopsis T6K22.50 protein. Previously, T6K22.50 has been shown to be a homologue of phosphosulfolactate synthase from Methanococcus jannaschii, a hyperthermophilic methane-producing bacterium. Here we tentatively named T6K22.50 as phosphosulfolactate synthase-like (PSL) protein. We found that homologues of PSL cDNA existed in various flowering plants by BLAST search of EST databases. In Arabidopsis or rice genome, only one copy of PSL genes is present. The Arabidopsis and rice PSL genes were also inducible by HS treatment, suggesting that PSL might be a novel plant HSP. Like many other HSPs, such as tom66 (a class I small HSP), expression of tomato PSL seems to be mainly associated with fruit development under non-HS conditions. The function of PSL will be discussed.
