Poster: Late and Moved Abstracts

Abs # 1427: A proteome analysis of Arabidopsis thaliana treated with the heavy metals cadmium and copper

Presenter:	Kung, Cheng-Che S., sagekung@gate.sinica.edu.tw
Authors	Kung, Cheng-Che S. (A)   Wu, Hsiu-Chung  (A)   Chiang, Huai-Chih  (A)   Yeh, Kuo-Chen  (A)
Affiliations:	(A): Institute of BioAgricultural Sciences, Academia Sinica

It is generally believed that plant have evolved a suite of homeostatic mechanisms to maintain precise concentrations of both essential and nonessential heavy metals. To better understand the molecular mechanisms of heavy metals tolerance and/or accumulation in plants, we launched proteome analyses of the model plant Arabidopsis in response to the heavy metal treatments. Seedlings were treated with various concentrations of cadmium or copper for a series of time periods. The protein profiles of Arabidopsis before and after heavy metal treatments were resolved on 2-dimensional PAGE. Proteins exhibiting differential expression patterns were visualized, excised and identified by peptide mass fingerprint (PMF) or MS/MS analyses. Identities of these differentially expressed proteins and their potential roles in plants under heavy metal stress will be discussed.