Minisymposium 26: Proteomics
Abs #
47004: Mining the plant cell wall proteome: new tools, pathways and extracellular processes
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Presenter: |
Rose, J. K., jr286@cornell.edu | Authors | Rose, J. K. (A) Lee, S-J (A) Lee, J-M (B) Kim, B-D (B) Catala, C (A) Saravanan, R S. (A) Yamane, H (A) Gee, C T. (A) | | Affiliations: |
(A): Department of Plant Biology, Cornell University, Ithaca , NY, USA
(B): Center for Plant Molecular Genetics and Breeding Research, Seoul National University, Seoul, Korea
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| Web Site: | http://labs.plantbio.cornell.edu/rose/ | |
The plant cell wall plays many critical roles in diverse aspects of growth and development. In addition to providing a structural support for the protoplast, thereby regulating cell size and shape, the wall and apoplast collectively represent the interface with the environment and are the boundary across which much vital information must be transmitted. Despite its fundamental importance, many aspects of plant wall structure and function remain mysterious. This is exemplified by the observation that, in comparison with other subcellular compartments, the proteome of the cell wall/apoplast is poorly defined and a substantial proportion of extracellular proteins remain to be identified.
We have been developing a multi-tiered proteomics program to provide insight into the qualitative and quantitative complexity of the plant cell wall, or apoplastic, proteome. A combination of experimental techniques coupled with in silico analyses are being used to identify and characterize suites of cell wall localized proteins and their cognate genes, many of which are unreported. The results to date include the detection of proteins with unusual modular structures, new sets of proteins associated with extracellular and transmembrane signaling, metabolic pathways that have not previously been observed in the apoplast and polypeptides that have been described as regulating cell fate in animals but that have never been observed in plants. Taken together, these strategies are providing a wealth of information that places the wall at an even more central position in the biology and biochemistry of the plant cell.
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