Poster: Temperature responses

Abs # 114: Metabolite Profiling of Arabidopsis Responses to Temperature Shock

Presenter:	Kaplan, Fatma , fkaplan@mail.ufl.edu
Authors	Kaplan, Fatma  (A)   Kopka, Joachim  (C)   Gatzke, Nicole  (C)   Zhao, Wei  (B)   Guy, Charles L. (A)
Affiliations:	(A): Plant Molecular & Cellular Biology Program, Department of Environmental Horticulture, University of Florida (B): Department of Statistics, University of Florida (C): Max Planck Institute of Molecular Plant Physiology

Metabolite profiling analysis was performed to identify compounds that may be involved with the induction of acquired thermotolerance in response to heat shock (40 C for 5, 15, 30, 60, 120 and 240 min), and with the induction of acquired freezing tolerance in response to cold acclimation (4 C for 1, 4,12, 24, 48, 96 h). Polar metabolite analyses were done using a GC-MS based platform. Approximately 420 unique compounds were resolved in the analysis. In addition to metabolites related to primary and secondary metabolism, accumulations of well-known compatible solutes, like proline and raffinose, were observed in response to both heat and cold shock. This presentation will highlight selected examples of novel metabolite responses to heat and cold shock. These findings will help to broaden our perspective, at the metabolite level, of plant responses to temperature extremes.