Poster: Temperature responses
Abs #
115: Induction of glycinebetaine in barley plants under heat stress.
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Presenter: |
Uchida, Akio , i021005d@mbox.nagoya-u.ac.jp |
Authors | Uchida, Akio (A) Shi, Weiming (A) Okamoto, Masanori (A) Koshiba, Tomokazu (A) Hibino, Takashi (A) Ichihara, Shigeyuki (A) Takabe, Teruhiro (A) Takabe, Tetsuko (A) | | Affiliations: |
(A): Graduate School of Bioagricultural Science, Nagoya University
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Glycinebetaine accumulation was characterized in winter barley seedlings grown at normal (25oC) and elevated temperatures (32, 37 or 42oC). Glycinebetaine levels remained around 5 μmol/gDW at 25oC, whereas they increased gradually up to 30 μmol/gDW during 1-‚S d under heat stress at 37oC. Then the level increased more after 6 d and run up to about 70 μmol/gDW at 37oC, although glycinebetaine did not increase at 42oC at all, suggesting too severe heat stress for barley plants. The level of betaine aldehyde dehydrogenase protein, a marker enzyme of glycinebetaine synthesis in barley, also increased in 8 h and reached at the maximum level around 1 d at 37oC. On the other hand, H2O2 and abscisic acid increased temporary before accumulation of mRNA for betaine aldehyde dehydrogenase. The level of mRNA for betaine aldehyde dehydrogenase was increased in 10 h. We found that the levels of betaine aldehyde dehydrogenase protein and its mRNA for glycinebetaine synthesis is induced by H2O2 and abscisic acid under heat stress at 37oC.
