Poster: Temperature responses
Abs #
134: Analysis of temperature stress Responses in Solanum tuberosum (potato) and other Solanaceae by comparative and functional genomics approaches
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Presenter: |
Rensink, Willem A, wrensink@tigr.org |
Authors | Rensink, Willem A (A) Iobst, Stacey (A) Hart, Amy (A) Stegalkina, Svetlana (A) Liu, Jia (A) Buell, Robin C (A) | | Affiliations: |
(A): TIGR, The Institute for Genomic Research
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| Web Site: | http://www.tigr.org/tdb/potato/ | |
The Solanaceae family represents a range of phenotypically diverse species that includes several important vegetable crops such as potato, tomato, pepper, and eggplant. We used the functional genomic resources such as EST sequences and microarrays generated for Solanum tuberosum (potato) to analyze the different species within the Solanaceae family. First, we performed a survey of the sequence similarity between potato, tomato, pepper, eggplant, tobacco and petunia using comparative genomic hybridizations (CGH) on potato cDNA microarrays. Combined with RNA expression data we show the value of potato resources as a model for Solanaceae species.
In order to identify genes involved in the abiotic stress response, a series of reference experiments for the transcriptome changes were performed. Potato plants were grown under controlled conditions and heat, cold or salt stress was applied. Samples were collected at 3, 9 and 27 hours both from leaves and roots. The experiment was repeated three times. The resulting expression data will be used to identify and classify the stress response genes.
To further identify the important genes in the heat stress response, 12 different wild potato species were selected based on the heat stress descriptors, from sensitive to resistant and subjected to heat stress. The expression profiles of the different wild potato species will be compared among each other and the reference datasets. In parallel, we surveyed six other Solanaceae species under temperature and other abiotic stresses enabling us to make a comparative analysis of the stress responses in the different species. All gene expression data will be made publicly available through the TIGR Solanaceae Gene Expression Database (http://www.tigr.org/tdb/potato/SGED_index2.shtml).
