Poster: Temperature responses

Abs # 137: Cis-Element Patterns of Temperature Shock Genes of Arabidopsis

Presenter:	Kaplan, Fatma , fkaplan@ufl.edu
Authors	Kaplan, Fatma  (A)   Sung, Dong Yul  (B)   Popp, Mick  (C)   Zhao, Wei  (D)   Guy, Charles  (A)
Affiliations:	(A): Plant Molecular & Cellular Biology Program, Department of Environmental Horticulture, University of Florida (B): Division of Biological Sciences, Cell and Developmental Biology Section, and Center for Molecular Genetics, University of California (C): UFSCC/ICBR Microarray Core, University of Florida (D): Department of Statistics, University of Florida

Transcript profiling analysis was conducted to search for genes associated with both acquired thermotolerance in response to heat shock (40 C for 5, 15, 30, 60, 120 and 240 min), and acquired freezing tolerance in response to cold acclimation (4 C for 1, 4,12, 24, 48, 96 h). Global transcript profiling was done using the Affymetrix ATH1 gene chip. Signals for 16,475 probe sets were subjected to ANOVA and Kruskal-Wallis tests. Based on statistical analyses, 1,078 genes were induced specifically in response to cold shock, and did not show significant responses to heat shock. Similarly, 900 genes showed induction specifically in response to heat shock, and did not show significant response to cold shock. Interestingly, a very small number of genes showed the same type of behavior under both cold and heat shock conditions. The promoter regions of the cold and heat specific genes as well as those of common response genes will be analyzed in an effort to reveal common temperature shock, and cold and heat shock specific networks. These findings will help to broaden our perspective of plant responses to temperature extremes at the gene expression level.