Poster: Temperature responses

Abs # 148: Molecular approach of new type thermo-sensitive genic male-sterile gene in rice (Oryza sativa L.)

Presenter:	Dong Sun, Lee , dong_east@yumail.ac.kr
Authors	Hak Soo, Suh  (A)   Li Juan, Chen  (B)   Sang Gu, Kang  (C)   Dong Sun, Lee  (D)
Affiliations:	(A): College of Natural Resources, Yeungnam University (B): Rice Research Institue, Yunnan Agricultural University (C): College of Biotechnoloty, Yeungnam University (D): Institute of Biotechnolotgy, Yeungnam University

Genetic male sterility has great potential in hybrid seed production. The two-line breeding system by using temperature-sensitive genetic male sterility (TGMS) has been discovered and successfully developed as a breeding strategy in rice. One TGMS gene was investigated by a spontaneous japonica TGMS line, Sokcho ms1, which was complete sterile when the temperature was higher than 27^oC, and lower than 25^oC during the differentiation stage of the spikelets, but fertile when the temperature was between 25-27^oC. Genetic analysis and molecular mapping based on simple sequence-repeat (SSR), sequence tagged site (STS), and cleaved amplified polymorphic sequence (CAPS) maps revealed a single recessive gene locus involved the control of genetic male sterility in Sokcho ms1. A new TGMS gene, tms6(t), was mapped primarily to the long arm of chromosome 5 of Oryza sativa at the interval between markers RM3351 (0.1 cM) and E60663 (2.0 cM) by using an F2 population derived from a cross between Sokcho ms1 and a fertile indica variety Neda. It was found that tms6(t) appeared to be differ from the other mapped TGMS genes in rice.