Poster: Water relations
Abs #
155: Eceriferum25 reveals a new locus associated with both cutin and cuticular wax production in Arabidopsis
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Presenter: |
Goodwin, S. Mark, goodwism@purdue.edu | Authors | Goodwin, S. Mark (A) Chen, Xinbo (A) Rahman, Musrur (B) Krochko, Joan (B) Jenks, Matthew (A) | | Affiliations: |
(A): Horticulture and Landscape Architecture, Purdue University, West Lafayette, Indiana 47907
(B): Plant Biotechnology Institute, National Research Council, Saskatoon, Saskatchewan, Canada S7N 0W9
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The plant cuticle forms the outermost barrier over essentially all aerial plant parts, including those of Arabidopsis, and plays an important role in plant drought resistance. SSLP and CAPS markers were used to map the CER25 locus to a position at 10.8 cM on chromosome 1. Total wax amount on cer25 inflorescence stems was 2.2 fold lower than on stems of wildtype, with reductions in all wax classes and individual constituents. Leaf wax of cer25 did not differ significantly from leaf wax of wildtype. Total stem cutin amount on cer25 stems was over 3.5 fold lower than in wildtype. The proportion of the most abundant cutin monomer, hexadecane-1,16-dioic acid, decreased from 44.3 % in wildtype to 21.7 % in cer25, and 10,16-dihydroxy hexadecanoic acid decreased from 15.0 to 5.3 % respectively. All other monomers except one minor constituent were also reduced. The ratio of cutin to the non-depolymerizable cutan in cer25 stem cuticle was 35.4 % and 64.6 %, respectively, which is unchanged from wildtype. The weight of isolated cer25 stem cuticle membrane was 3.1 mg cm-2 whereas wildtype was 3.9 mg cm-2. Whole flowering plant in-pot transpiration rates were higher for cer25 than wildtype. Cer25 light and dark transpiration rates were 44.9% and 76.1% higher, respectively, than for wildtype. Besides cuticle lipid alterations, the cer25 mutant displayed delayed bolting, reduced inflorescence height, conditional sterility, and reduced leaf stomatal index. Mutation of the CER25 locus reveals a unique association between plant cuticle lipid production and plant drought resistance and development.
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