Poster: Water relations
Abs #
159: Microarray analysis of flood stress responsive genes in rice.
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Presenter: |
Yamada, Hitomi , yhitomi@nias.affrc.go.jp |
Authors | Yamada, Hitomi (A) Ooka, Hisako (A) Tasaki, Kimihisa (B) Lee, Jung-Sook (C) Satoh, Kouji (A) Kikuchi, Shoshi (A) | | Affiliations: |
(A): National Institute of Agrobiological Sciences
(B): Tochigi Prefectural Agricultual Experiment Station
(C): Rural Development Administration
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Plants are exposed to many varieties of environmental stresses, including cold, drought, salt, osmotic and flood stress etc. Global expression profiles of the flood stresses were analyzed using 22K rice oligo microarray. We constructed two kinds of flood stress treatment models, Flood Standing (FS) and Flood Lay Down (FD). Combined results from two kinds of flood stresses revealed 1,166 genes with greater than 2-fold change over (Up regulation) or less than 0.5-fold change over (Down regulation) control. Numbers of genes after each stress treatment (24hr) is as follows, 962 genes (5%) were changed in FS and 469 genes (2.3%) were in FD. Commonly up-regulated genes in both two flood stresses were 130 genes, including peroxidase and glucose biosynthesis etc. Commonly down-regulated genes were 132 genes, including nitrate reductase and transcription factor-like gene etc. We also showed results in the gene expression analysis of other stress treatment of rice. Cluster analysis of the common responsive genes in both two kinds of flood stresses has done with otheres four stress, cold, drought, salt and osmotic stress treatments. In 130 up-regulated genes in both two flood stresses, 48 genes cross-talked with other stress. Three genes out of 48 genes were up-regulated in all stress treatments. They are candidates of the common stress responsive genes. Remaining 82 genes didnft cross-talk with other stress. They are candidates of the flood stress specific genes.
