Poster: Secondary metabolism

Abs # 261: Enhanced production of paclitaxel in sodium butyrate-supplemented suspension culture of Taxus chinensis

Presenter:	Kim, Chang-Heon , chkim@genex.co.kr
Authors	Kim, Chang-Heon  (A)   Kim, Jin-Ah  (A)   Son, Joo-Sun  (A)   Song, Jai Young  (A)   Choi, Ho Joon  (A)
Affiliations:	(A): Samyang Genex Biotech Research Institute
Web Site:	http://www.genex.co.kr

Elicitors in plants can promote secondary metabolites production followed by triggering the defense mechanism, induction of signal transduction and modulation of cell cycle. And it was reported that plant-microorganism interactions involve histone acetylation by inhibition of histone deacetylase. Sodium butyrate, an inhibitor of histone deacetylase, has various biological activities on both animal and plant cells. To the best of our knowledge, however, there have been no reports to date on the stimulation of secondary metabolites in plant cell culture by sodium butylate. In this work, we report that sodium butyrate stimulates production of paclitaxel and other taxanes in Taxus chinensis cell culture. Productions of paclitaxel was increased about 2 folds when 1 mM of sodium butyrate was added to the culture at 7 days after incubation. Paclitaxel and other taxane production were further increased about 1.6 to 9 folds with repeated treatment of sodium butyrate. Further experiments are undergoing to elucidate the possible mechanism of sodium butyrate-mediated enhancement of secondary metabolites.