Poster: Reproductive development
Abs #
353: Large scale analysis of gene expression in the tobacco stigma/style – The TOBEST project
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Presenter: |
Goldman, Maria Helena S., mgoldman@ffclrp.usp.br |
Authors | Goldman, Maria Helena S. (A) Quiapim, Andréa C. (A) Malavazi, Iran (B) daSilva, Idalete (A) Molfetta, Jeanne B. (A) Vitorelli, Patrícia M. (A) Goldman, Gustavo H. (B) | | Affiliations: |
(A): FFCLRP/University of Sao Paulo Department of Biology
(B): FCFRP/University of Sao Paulo Department of Pharmaceutical Sciences
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| Web Site: | http://143.107.203.68/Mhelena/default.html | |
The stigma/style is composed of specialized tissues with important roles in sexual reproduction. The success of the pollination process depends on pollen-pistil interactions that take place in these specialized tissues. To better understand the development and function of the stigma/style, we have constructed a tobacco stigma/style cDNA library and sequenced from the 5’-end 10,637 ESTs with at least 300 bp with Phred/Phrap quality above 20. After CAP3 clustering, we have identified 4,930 clusters (1,671 contigs and 3,259 singlets). The clusters were analyzed for homology to other gene sequences using the BLAST programs. Twenty-four percent of the sequences did not show significant similarity to sequences present in the public databases and twenty-three percent showed similarity to hypothetical proteins. Fifty-three percent were organized into functional groups. Sequences with significant similarity to AGAMOUS, FRUITFUL, CRABS CLAW, ETTIN, TOUSLED, CLAVATA 1, ERECTA, PINOID, SPATULA and KANADI were identified in the TOBEST database. Despite the fact that APETALA1, APETALA2 and PISTILLATA were not expected to be expressed in pistils, we also found ESTs with significant similarity to these sequences. Representative clones of 1,408 clusters were used to prepare macroarray membranes. The membranes were hybridized to probes prepared from stigma/style and vegetative organs (root, stem, leaf, sepal and petal). From the 616 clones (singlets) already analyzed, we were able to identify 71 (11.5%) clones preferentially expressed in stigma/style. Among these clones, there were some encoding RNA binding proteins, transcription factors, proteins induced during stress responses, chaperonins and proteins involved in signal transduction.
Financial support: CNPq and FAPESP, Brazil
