Poster: Plant-pathogen interactions
Abs #
552: Study of the disease resistance mechanism using AFLP-transcript profiling in Phaseolus vulgaris: cloning of mRNA induced by Xanthomonas axonopodis
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Presenter: |
Chaudhary, Sarita , chaudharys@agr.gc.ca |
Authors | Chaudhary, Sarita (A) Yu, Kangfu (A) Park, Soon (A) | | Affiliations: |
(A): Greenhouse and Processing Crops Research Center, Agriculture and Agri-Food Canada
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Plant diseases are one of the major biotic constraints to crop productivity and production throughout the world. Common bacterial blight (CBB) caused by Xanthomonas axonopodis pv. Phaseoli (Xap) is one of the most important diseases affecting bean production world-wide. Advances in molecular biology have paved the way for the genetic dissection of disease resistance. Disease resistance genes have been successfully isolated in a variety of species including soybean, tomato, potato and rice. However, no CBB resistant gene has been cloned in beans (Phaseolus vulgaris) to date. To understand the molecular bases of disease resistance in beans during CBB infection we used cDNA-AFLP to identify differentially expressed transcripts from P. vulgaris leaves subjected to Xap infection.
Common bean line HR45 is resistant to CBB. Leaf tissue was collected from infected HR45 plants at different time points after pathogen inoculation. The cDNA-AFLP fragments from infected and non-infected plants were separated in parallel by gel electrophoresis. The AFLP bands induced or repressed specifically by Xap were identified. Thirty seven different primer combinations were tested and 3052 transcript derived fragments (TDF) were obtained. One hundred and five fragments (3.44 %) were induced or repressed after inoculation with Xap. Here we report the cloning and characterization of about 20 differential transcript derived fragments. Putative function of these TDF in plant defence will be discussed. A cDNA library has been generated by inoculating HR45 with Xap. The cDNA library will be screened to get corresponding cDNAs. The cDNA associated with Xap resistance will further be investigated for molecular marker development and utilization in marker-assisted selection programs in bean breeding.
