Poster: Plant-pathogen interactions

Abs # 565: Characterization of two Dc3000 effector proteins: HopPtoK and HopPtoJ

Presenter:	Robert-Seilaniantz, Alexandre , ars4343@ksu.edu
Authors	Robert-Seilaniantz, Alexandre  (A)   Zhou, Jian-Min  (A)   Tang, Xiaoyan  (A)
Affiliations:	(A): Kansas state university

Pseudomonas syringae pv tomato DC3000 has emerged as a model for plant pathogenic bacteria because it can infect both tomato and Arabidopsis. Its ability to cause disease relies on the presence of genes encoding a type three-secretion system (TTSS) and effector proteins that are injected through the TTSS system into the plant cell. The recent complete sequence of DC3000 genome and functional analysis showed more than 36 effectors. The effector proteins show diverse sequence characteristics, but little is known about their virulence function and their general behavior in the plant cell. To assess the function of these effector proteins, we expressed them in Arabidopsis transgenic plants under the control of an oestrogen inducible promoter. These plants have a Columbia genetic background and Rap2.6:LUC reporter gene that can be induced by DC3000 infection. We will report the characterization of two effectors proteins: HopPtoJ and HopPtoK.