Poster: Plant-pathogen interactions
Abs #
569: Expression of Defense-related Genes Associated with Soybean-Phytophthora sojae Interactions
|
|
Presenter: |
Zhou, Lecong , lzhou@vbi.vt.edu |
Authors | Zhou, Lecong (A) (B) Tripathy, Sucheta (A) Torto-Alalibo, Trudy (A) Qutob, Dinah (C) Gijzen, Mark (C) Maroof, Saghai M.A. (B) Tyler, Brett M. (A) | | Affiliations: |
(A): Virginia Bioinformatics Institute, Virginia Polytechnic Institute and State University
(B): Department of Crop and Soil Environmental Sciences, Virginia Polytechnic Institute and State University
(C): Agriculture and Agri-Food Canada
|
| Web Site: | http://www.vbi.vt.edu | |
Soybean stem and root rot is one of the most devastating diseases caused by Phytophthora sojae. A compatible soybean infection library has been constructed from which a total of 3,744 soybean ESTs were generated. This compatible library was compared for gene expression profiles to an incompatible interaction library from the public soybean EST project, which contains 4,675 verified soybean ESTs. A total of 2,229 and 2,871 unigenes have been clustered and assembled from the compatible and incompatible libraries, respectively. A putative function was assigned to each unigene by standalone BLASTX against the non-redundant (NR) protein database. Genes that are composed of five or more ESTs are considered as highly expressed in response to P. sojae infection. Putative defense related (DR) genes were extracted and categorized. The percentages of each DR gene category to the entire unigene set were very similar in the two libraries, but some components of individual DR gene categories differed, indicating that the compatible and incompatible interactions share generally similar responses but some responses were specific to compatibility or incompatibility. The unigene sets that appeared to be specific to the compatible or incompatible interactions were extracted by similarity search against 328,793 soybean ESTs derived from 114 non-P. sojae challenged soybean cDNA libraries. The establishment of P. sojae infection-specific unigene set narrows the scope for searching for novel DR genes. The collection of ESTs (available at http://staff.vbi.vt.edu/estap/) and unigenes from P. sojae infection libraries will provide a useful resource for study of soybean defense responses to pathogen attack.
